ELISA is a simple, popular as well as cost-efficient technique of detection. Read this blog and know the tips to select the right ELISA kits suppliers in India. 

The enzyme-linked immunosorbent assay uses antibodies and enzymes to identify a particular substance in a liquid sample. These include culture supernatant, serum/plasma and cell/tissues. Moreover, the ELISA kit providers in India state that it is a wet lab-type analytic method. 

6 Tips To Choose The Right ELISA Kit 

• The Species Researched

These samples include classic models such as rats, mice and humans which are relatively easier to find. However, if the sample belongs to a non-classical model, there are very few numbers of commercial ELISA kits available. In this situation, you might need to select a kit validated on these species. Further, this presents homology with the species of the sample. 

• The Detected Analyte 

You must have a clear understanding of what kind of analyte to choose. For identifying large proteins with several epitopes, the most suitable option is sandwich ELISA. A competitive ELISA is perfect for the detection of small molecules such as hapten. 

• Purpose Of The Analysis

ELISA tools are useful for both quantitative and qualitative analyses. Qualitative ELISA offers a simple negative or positive test result for the sample. On the other hand, quantitative ELISA presents the concentration of the analyte in a sample with the help of a standard curve. Depending on the purpose of the analysis, you have to choose between quantitative or qualitative ELISA. 

• Antibodies Type

You can consult any ELISA kit supplier on what kind of antibodies you want to use in the kit. Whether you prefer monoclonal antibodies or polyclonal antibodies. In the case of a sandwich ELISA, using a polyclonal antibody is sometimes beneficial for capturing. 

• Sample Size

The ideal size of samples in ELISA kits must be between 100ul down to 10ul sample. If you have a very small amount of samples or are an expensive one, it’s better to opt for ELISA kits that demand less sample amount. 

• Sensitivity Requirement

ELISA kits having a broad range of detection are always a better option. If you have a sample with low analyte concentration, it’s better to choose ELISA kits with high sensitivity. Dilution of samples may become necessary when the concentration of the analyte is too high. Moreover, it will enable the adaption to the ELISA Kits’ detention range. 

Conclusion

Elisa Kit For Food Safety & Drug Residues is essential for every biological research. Avail yourself of quality ELISA kits with Biochain. Contact the biggest Thermo fisher scientific products distributors today and get ELISA kits available 24*7 with the fastest delivery.