Know how the ELISA kits can be a major help in detecting antigens and also, antibodies . Keep reading the blog to know more about it. 

ELISA is a plate-based assay technique that finds use in a variety of applications. Also, it involves the use of a solid surface, usually a polystyrene multi well plate, which distinguishes ELISA from other antibody-based assays.

Our Precoated ELISA kits  analyte in a sample is immobilized on the solid surface, while other components in the sample need a detergent solution for removal. In this way, the solid surface enables the separation of the analyte from the sample. After immobilization, the addition of the detection antibody linked to an enzyme, forming an antigen-antibody complex completes the process. Finally, the experts add an enzymatic substrate to react with the enzyme. The enzymatic reaction generates a visible signal, usually color change, which is easily measurable. 

The Five-Step Procedure Of An ELISA

Enzyme-linked immunosorbent assay is a biochemical technique that finds applications including, microbiology, blood screening, veterinary, and also, immunology.  It is also used for quality control checks in food industries. ELISAs depend on characteristic antibodies to fix the target antigen. 

1. Primary Antigen (or antibody) helps to coat microtiter plate wells

2. The next step is to wash the plates to remove unbound antibodies. False-positive results need to be reduced and also, this can happen by blocking unbound sites. 

3. Addition of a selected enzyme-conjugated primary detection antibody.

4. Addition of secondary antibody conjugated to an enzyme (such as anti-mouse IgG). The reaction of this enzyme with any substrate present produces a colored product indicating a positive reaction.

To measure the results, a spectroscope is an instrument. Some assays include a Sample Addition Monitor system that shows a colour change at each step of the process. This can be important when automated liquid handling systems come into play. 

What Are The Factors For Choosing The Right ELISA Kit?

• The species studied

• The analyte detected

• Purpose of the analysis

• Type of antibodies

• Requirement of sensitivity

• Sample size

• Recovery and linearity data

• Detection system

• The experimental protocol

Conclusion

BioChain Incorporated is one of the finest Elisa Kit suppliers, where  shipping, custom processes, fast delivery, and culture to save your time and also, stress. We offer the kit  starting at Rs. 7800/-. These kits allow specific, quantitative measurements of disease-related proteins, including cytokines, chemokines, and also, signaling targets.  Kits are applied for Human Rat , Mouse & samples for serum, plasma, tissue homogenates, cell culture supernates