Human Hepatitis E virus antibody(IgG)ELISA Kit
Product Details
| Description |
This Human Hepatitis E virus antibody (IgG) ELISA Kit is designed to qualitatively detect the HEV IgG antibody in the serum and plasma. It employs the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with HEV antigen. Samples or standards are pipetted into the wells with anti-human IgG conjugated HRP. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of human HEV IgG antibody bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm.The valence of human HEV IgG antibody in the samples is determined by referring to the negative control. It indicates the presence of HEV IgG antibody if the O.D. (optical density) of sample is greater than or equal to the cutoff value (average Negative Control O.D. value plus 0.10). There is no HEV IgG antibody present in the sample if the O.D. is less t
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| Target Name |
Hepatitis E virus antibody(IgG) |
| Abbreviation |
HEV Ab (IgG) |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma |
| Detection Range |
Request Information |
| Sensitivity |
Request Information |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Microbiology |
| Assay Principle |
qualitative |
| Measurement |
Indirect |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Typical Data |
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| Test parameter |
specification |
test result |
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| Positive control |
>0.6 |
0.799 |
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| Negative control |
0.046 |
| Positive rate |
10，Positive |
100% |
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| Negative rate |
10，Negative |
100% |
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| Materials provided |
- A 96-well Assay plate --The 96-well plate has been pre-coated with HEV antigen
- Negative Control (1 x 0.4ml) --Eliminate false positive
- Positive Control (1 x 0.4ml) --Used to evaluate the validity, stability, and comparability of experimental results.
- HRP-conjugated IgG antibody(1 x 12ml) --Bind to the anti-HEV IgG antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
- Sample Diluent (1x 12ml) --Dilute the sample to an appropriate concentration.
- Wash Buffer (20x concentrate) (1 x 50 ml) --Wash away unbound or free substances.
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plates
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
