Bovine adiponectin,ADP ELISA Kit
Product Details
| Target Name |
adiponectin, C1Q and collagen domain containing |
| Alternative Names |
ADIPOQ ELISA kit; ACRP30 ELISA kit; APM1Adiponectin ELISA kit; 30 kDa adipocyte complement-related protein ELISA kit; Adipocyte complement-related 30 kDa protein ELISA kit; ACRP30 ELISA kit; Adipocyte ELISA kit; C1q and collagen domain-containing protein ELISA kit; Adipose most abundant gene transcript 1 protein ELISA kit; apM-1 ELISA kit |
| Abbreviation |
ADIPOQ |
| Species |
Bos taurus (Bovine) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
3.125 μg/mL-50 μg/mL |
| Sensitivity |
3.125 μg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
87-97 |
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| 1:2 |
Average % |
97 |
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| Range % |
93-104 |
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| 1:4 |
Average % |
85 |
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| Range % |
82-91 |
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| 1:8 |
Average % |
90 |
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| Range % |
85-97 |
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| Recovery |
| The recovery of bovine ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-99 |
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| EDTA plasma (n=4) |
98 |
94-103 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/ml |
OD1 |
OD2 |
Average |
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| 50 |
0.212 |
0.207 |
0.210 |
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| 25 |
0.345 |
0.332 |
0.339 |
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| 12.5 |
0.589 |
0.607 |
0.598 |
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| 6.25 |
1.077 |
1.106 |
1.092 |
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| 3.125 |
2.214 |
2.123 |
2.169 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
