Bovine Caspase 7(Casp-7) ELISA Kit
Product Details
| Target Name |
Caspase 7(Casp-7) |
| Abbreviation |
Casp7 |
| Species |
Bos taurus (Bovine) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine Casp-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
85-101 |
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| 1:2 |
Average % |
95 |
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| Range % |
88-102 |
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| 1:4 |
Average % |
89 |
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| Range % |
83-100 |
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| 1:8 |
Average % |
93 |
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| Range % |
89-106 |
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| Recovery |
| The recovery of bovine Casp-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
90-112 |
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| EDTA plasma (n=4) |
93 |
84-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.546 |
2.457 |
2.502 |
2.369 |
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| 20 |
1.883 |
1.905 |
1.894 |
1.761 |
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| 10 |
1.136 |
1.124 |
1.130 |
0.997 |
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| 5 |
0.726 |
0.711 |
0.719 |
0.586 |
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| 2.5 |
0.407 |
0.397 |
0.402 |
0.269 |
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| 1.25 |
0.305 |
0.313 |
0.309 |
0.176 |
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| 0.625 |
0.196 |
0.204 |
0.200 |
0.067 |
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| 0 |
0.130 |
0.136 |
0.133 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
