Bovine Heat Shock Protein 70,HSP-70 ELISA Kit
Product Details
| Target Name |
Heat Shock Protein 70,HSP-70 |
| Abbreviation |
HSP70 |
| Species |
Bos taurus (Bovine) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
1.25 ng/mL-20 ng/mL |
| Sensitivity |
1.25 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
91-101 |
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| 1:2 |
Average % |
90 |
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| Range % |
85-94 |
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| 1:4 |
Average % |
98 |
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| Range % |
92-103 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of bovine HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
84 |
80-89 |
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| EDTA plasma (n=4) |
95 |
91-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 20 |
0.135 |
0.131 |
0.133 |
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| 10 |
0.219 |
0.231 |
0.225 |
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| 5 |
0.427 |
0.401 |
0.414 |
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| 2.5 |
0.747 |
0.792 |
0.770 |
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| 1.25 |
1.724 |
1.626 |
1.675 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
