Bovine Insulin-like growth factor 1,IGF-1 ELISA Kit
Product Details
| Target Name |
insulin-like growth factor 1 (somatomedin C) |
| Alternative Names |
IGF1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit |
| Abbreviation |
IGF1 |
| Species |
Bos taurus (Bovine) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.75 ng/mL-60 ng/mL |
| Sensitivity |
0.94 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
86-94 |
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| 1:2 |
Average % |
96 |
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| Range % |
92-100 |
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| 1:4 |
Average % |
83 |
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| Range % |
80-86 |
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| 1:8 |
Average % |
91 |
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| Range % |
83-98 |
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| Recovery |
| The recovery of bovine IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
87-99 |
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| EDTA plasma (n=4) |
93 |
91-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 60 |
0.387 |
0.389 |
0.388 |
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| 22.5 |
0.532 |
0.567 |
0.550 |
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| 11.25 |
0.764 |
0.789 |
0.777 |
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| 3.75 |
1.149 |
1.168 |
1.159 |
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| 0.75 |
1.636 |
1.638 |
1.637 |
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| 0 |
2.105 |
2.118 |
2.112 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
