Bovine Interferon-Tau ELISA Kit
Product Details
| Target Name |
Interferon-Tau |
| Alternative Names |
IFNT1 ELISA Kit; Interferon tau-1 ELISA Kit; IFN-tau-1 ELISA Kit; Antiluteolysin ELISA Kit; Trophoblast antiluteolytic protein ELISA Kit; Trophoblast protein 1 ELISA Kit; TP-1 ELISA Kit; Trophoblastin ELISA Kit |
| Abbreviation |
IFN-tau |
| Species |
Bos taurus (Bovine) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
6.25 pg/mL-400 pg/mL |
| Sensitivity |
1.56 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine interferon-tau in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
82-92 |
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| 1:2 |
Average % |
98 |
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| Range % |
94-101 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-98 |
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| 1:8 |
Average % |
95 |
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| Range % |
87-98 |
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| Recovery |
| The recovery of bovine interferon-tau spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-96 |
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| EDTA plasma (n=4) |
96 |
91-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.587 |
2.558 |
2.573 |
2.485 |
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| 200 |
2.140 |
2.003 |
2.072 |
1.984 |
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| 100 |
1.644 |
1.621 |
1.633 |
1.545 |
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| 50 |
1.109 |
1.027 |
1.068 |
0.980 |
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| 25 |
0.698 |
0.687 |
0.693 |
0.605 |
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| 12.5 |
0.399 |
0.387 |
0.393 |
0.305 |
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| 6.25 |
0.242 |
0.231 |
0.237 |
0.149 |
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| 0 |
0.090 |
0.086 |
0.088 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
