Bovine Interleukin 1,IL-1 ELISA Kit
Product Details
| Target Name |
Interleukin 1,IL-1 |
| Alternative Names |
IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit |
| Abbreviation |
IL1A |
| Species |
Bos taurus (Bovine) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
250 pg/mL-4000 pg/mL |
| Sensitivity |
250 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-94 |
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| 1:2 |
Average % |
92 |
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| Range % |
87-97 |
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| 1:4 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:8 |
Average % |
98 |
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| Range % |
90-102 |
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| Recovery |
| The recovery of bovine IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-98 |
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| EDTA plasma (n=4) |
89 |
83-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 4000 |
0.147 |
0.141 |
0.144 |
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| 2000 |
0.296 |
0.279 |
0.288 |
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| 1000 |
0.431 |
0.467 |
0.449 |
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| 500 |
0.782 |
0.823 |
0.803 |
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| 250 |
1.862 |
1.826 |
1.844 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
