Bovine Interleukin 2,IL-2 ELISA Kit
Product Details
| Target Name |
interleukin 2 |
| Alternative Names |
IL2 ELISA kit; IL-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit |
| Abbreviation |
IL2 |
| Species |
Bos taurus (Bovine) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
81-90 |
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| 1:2 |
Average % |
100 |
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| Range % |
97-103 |
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| 1:4 |
Average % |
101 |
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| Range % |
98-105 |
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| 1:8 |
Average % |
94 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of bovine IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-97 |
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| EDTA plasma (n=4) |
96 |
92-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.151 |
2.099 |
2.125 |
2.040 |
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| 1000 |
1.683 |
1.656 |
1.670 |
1.585 |
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| 500 |
1.284 |
1.270 |
1.277 |
1.192 |
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| 250 |
0.846 |
0.833 |
0.840 |
0.755 |
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| 125 |
0.502 |
0.498 |
0.500 |
0.415 |
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| 62.5 |
0.367 |
0.356 |
0.362 |
0.277 |
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| 31.25 |
0.221 |
0.213 |
0.217 |
0.132 |
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| 0 |
0.086 |
0.084 |
0.085 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
