Bovine Interleukin 6,IL-6 ELISA Kit
Product Details
| Target Name |
interleukin 6 (interferon, beta 2) |
| Alternative Names |
IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit |
| Abbreviation |
IL6 |
| Uniprot No. |
P26892 |
| Species |
Bos taurus (Bovine) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
5 pg/mL-1000 pg/mL |
| Sensitivity |
2.5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
90-97 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
88-101 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-100 |
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| Recovery |
| The recovery of bovine IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
94-101 |
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| EDTA plasma (n=4) |
96 |
92-103 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 1000 |
0.071 |
0.075 |
0.073 |
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| 250 |
0.192 |
0.205 |
0.199 |
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| 80 |
0.421 |
0.451 |
0.436 |
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| 20 |
0.876 |
0.897 |
0.887 |
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| 5 |
1.345 |
1.316 |
1.331 |
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| 0 |
2.116 |
2.215 |
2.166 |
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Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
