Bovine Interleukin 8,IL-8 ELISA Kit
Product Details
| Target Name |
interleukin 8 |
| Alternative Names |
CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit |
| Abbreviation |
IL8 |
| Species |
Bos taurus (Bovine) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
50 pg/mL-2000 pg/mL |
| Sensitivity |
50 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
82-95 |
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| 1:2 |
Average % |
94 |
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| Range % |
86-100 |
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| 1:4 |
Average % |
88 |
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| Range % |
80-94 |
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| 1:8 |
Average % |
92 |
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| Range % |
86-100 |
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| Recovery |
| The recovery of bovine IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
100 |
95-106 |
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| EDTA plasma (n=4) |
98 |
94-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 0 |
2.655 |
2.514 |
2.585 |
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| 50 |
1.279 |
1.232 |
1.256 |
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| 150 |
0.611 |
0.638 |
0.625 |
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| 460 |
0.342 |
0.363 |
0.353 |
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| 1000 |
0.218 |
0.224 |
0.221 |
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| 2000 |
0.125 |
0.131 |
0.128 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
