Canine Matrix metalloproteinase 13,MMP-13 ELISA kit
Product Details
| Target Name |
Matrix metalloproteinase 13,MMP-13 |
| Abbreviation |
MMP-13 |
| Species |
Canis lupus familiaris (Dog) (Canis familiaris) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
88-99 |
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| 1:2 |
Average % |
99 |
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| Range % |
95-106 |
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| 1:4 |
Average % |
87 |
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| Range % |
84-92 |
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| 1:8 |
Average % |
91 |
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| Range % |
85-98 |
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| Recovery |
| The recovery of canine MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
87-95 |
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| EDTA plasma (n=4) |
88 |
85-92 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
3.160 |
3.255 |
3.208 |
3.107 |
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| 5 |
2.614 |
2.712 |
2.663 |
2.562 |
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| 2.5 |
1.784 |
1.932 |
1.858 |
1.757 |
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| 1.25 |
1.026 |
1.030 |
1.028 |
0.927 |
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| 0.625 |
0.478 |
0.487 |
0.483 |
0.382 |
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| 0.312 |
0.264 |
0.256 |
0.260 |
0.159 |
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| 0.156 |
0.156 |
0.166 |
0.161 |
0.060 |
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| 0 |
0.105 |
0.097 |
0.101 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
