Canine Matrix metalloproteinase 1,MMP-1 ELISA kit
Product Details
| Target Name |
Matrix metalloproteinase 1,MMP-1 |
| Abbreviation |
MMP-1 |
| Species |
Canis lupus familiaris (Dog) (Canis familiaris) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.18 ng/mL-12 ng/mL |
| Sensitivity |
0.04 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
85-102 |
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| 1:2 |
Average % |
89 |
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| Range % |
85-99 |
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| 1:4 |
Average % |
94 |
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| Range % |
85-97 |
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| 1:8 |
Average % |
90 |
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| Range % |
83-98 |
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| Recovery |
| The recovery of canine MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
82-98 |
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| EDTA plasma (n=4) |
97 |
89-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 12 |
2.309 |
2.326 |
2.318 |
2.114 |
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| 6 |
1.754 |
1.699 |
1.727 |
1.523 |
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| 3 |
1.301 |
1.338 |
1.320 |
1.116 |
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| 1.5 |
0.931 |
0.920 |
0.926 |
0.722 |
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| 0.75 |
0.573 |
0.585 |
0.579 |
0.375 |
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| 0.37 |
0.412 |
0.407 |
0.410 |
0.206 |
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| 0.18 |
0.299 |
0.303 |
0.301 |
0.097 |
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| 0 |
0.201 |
0.206 |
0.204 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
