Canine Prostaglandin E2,PG-E2 ELISA Kit
Product Details
| Target Name |
Prostaglandin E2,PG-E2 |
| Abbreviation |
PG-E2 |
| Species |
Canis lupus familiaris (Dog) (Canis familiaris) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
10 pg/mL-2000 pg/mL |
| Sensitivity |
5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of canine PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
90-104 |
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| 1:2 |
Average % |
95 |
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| Range % |
89-105 |
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| 1:4 |
Average % |
93 |
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| Range % |
87-100 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-101 |
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| Recovery |
| The recovery of canine PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
85-96 |
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| EDTA plasma (n=4) |
96 |
89-103 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 2000 |
0.187 |
0.174 |
0.181 |
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| 500 |
0.320 |
0.315 |
0.318 |
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| 160 |
0.517 |
0.547 |
0.532 |
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| 40 |
0.814 |
0.848 |
0.831 |
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| 10 |
1.316 |
1.374 |
1.345 |
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| 0 |
1.958 |
1.933 |
1.946 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
