Chicken Heat Shock Protein 70,Hsp-70 ELISA Kit
Product Details
| Target Name |
Heat Shock Protein 70,Hsp-70 |
| Alternative Names |
Heat shock 70 kDa protein ELISA Kit; HSP70 ELISA Kit |
| Abbreviation |
HSP70 |
| Species |
Gallus gallus (Chicken) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.25 ng/mL-50 ng/mL |
| Sensitivity |
0.125 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken Hsp-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
83-97 |
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| 1:2 |
Average % |
91 |
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| Range % |
85-98 |
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| 1:4 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:8 |
Average % |
93 |
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| Range % |
84-99 |
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| Recovery |
| The recovery of chicken Hsp-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
92-104 |
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| EDTA plasma (n=4) |
96 |
90-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 0 |
2.576 |
2.518 |
2.547 |
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| 0.25 |
1.415 |
1.388 |
1.402 |
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| 1 |
1.035 |
1.047 |
1.041 |
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| 4 |
0.610 |
0.598 |
0.604 |
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| 12.5 |
0.329 |
0.314 |
0.322 |
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| 50 |
0.173 |
0.175 |
0.174 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
