Chicken Interleukin 17,IL-17 ELISA Kit
Product Details
| Target Name |
Interleukin 17,IL-17 |
| Abbreviation |
IL17 |
| Species |
Gallus gallus (Chicken) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
150 pg/mL-6000 pg/mL |
| Sensitivity |
150 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
82-100 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:4 |
Average % |
96 |
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| Range % |
90-101 |
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| 1:8 |
Average % |
91 |
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| Range % |
85-98 |
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| Recovery |
| The recovery of chicken IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
85-96 |
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| EDTA plasma (n=4) |
92 |
86-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 0 |
1.914 |
2.027 |
1.971 |
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| 150 |
1.306 |
1.329 |
1.318 |
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| 450 |
1.039 |
1.102 |
1.071 |
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| 1380 |
0.755 |
0.702 |
0.729 |
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| 3000 |
0.484 |
0.462 |
0.473 |
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| 6000 |
0.351 |
0.348 |
0.350 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
