Chicken Interleukin 18,IL-18 ELISA KIT
Product Details
| Target Name |
interleukin 18 (interferon-gamma-inducing factor) |
| Alternative Names |
IL18 ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit |
| Abbreviation |
IL18 |
| Species |
Gallus gallus (Chicken) |
| Sample Types |
serum, plasma, tissue Homogenates |
| Detection Range |
20 pg/mL-500 pg/mL |
| Sensitivity |
1.95 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
89-102 |
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| 1:2 |
Average % |
90 |
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| Range % |
82-99 |
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| 1:4 |
Average % |
93 |
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| Range % |
85-102 |
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| 1:8 |
Average % |
96 |
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| Range % |
92-100 |
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| Recovery |
| The recovery of chicken IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
81-99 |
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| EDTA plasma (n=4) |
95 |
89-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
1.981 |
1.927 |
1.954 |
1.851 |
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| 250 |
1.334 |
1.374 |
1.354 |
1.251 |
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| 125 |
0.920 |
0.915 |
0.918 |
0.815 |
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| 62.5 |
0.619 |
0.643 |
0.631 |
0.528 |
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| 31.2 |
0.395 |
0.376 |
0.386 |
0.283 |
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| 15.6 |
0.290 |
0.306 |
0.298 |
0.195 |
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| 7.8 |
0.211 |
0.224 |
0.218 |
0.115 |
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| 0 |
0.102 |
0.103 |
0.103 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
