Chicken Interleukin 4,IL-4 ELISA Kit
Product Details
| Target Name |
Interleukin 4,IL-4 |
| Abbreviation |
IL-4 |
| Species |
Gallus gallus (Chicken) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
12.5 pg/mL-800 pg/mL |
| Sensitivity |
3.12 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
80-95 |
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| 1:2 |
Average % |
94 |
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| Range % |
88-101 |
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| 1:4 |
Average % |
93 |
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| Range % |
86-106 |
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| 1:8 |
Average % |
95 |
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| Range % |
82-100 |
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| Recovery |
| The recovery of chicken IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
90-97 |
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| EDTA plasma (n=4) |
92 |
85-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
1.726 |
1.825 |
1.776 |
1.665 |
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| 400 |
1.336 |
1.383 |
1.360 |
1.249 |
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| 200 |
1.060 |
0.981 |
1.021 |
0.910 |
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| 100 |
0.714 |
0.706 |
0.710 |
0.599 |
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| 50 |
0.481 |
0.475 |
0.478 |
0.367 |
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| 25 |
0.307 |
0.292 |
0.300 |
0.189 |
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| 12.5 |
0.227 |
0.214 |
0.221 |
0.110 |
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| 0 |
0.108 |
0.113 |
0.111 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
