Chicken Interleukin 6,IL-6 ELISA Kit
Product Details
| Target Name |
interleukin 6 (interferon, beta 2) |
| Alternative Names |
IL6 ELISA Kit; Interleukin-6 ELISA Kit; ChIL-6 ELISA Kit; IL-6 ELISA Kit |
| Abbreviation |
IL6 |
| Species |
Gallus gallus (Chicken) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
85-97 |
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| 1:2 |
Average % |
92 |
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| Range % |
85-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
89-100 |
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| 1:8 |
Average % |
92 |
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| Range % |
85-100 |
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| Recovery |
| The recovery of chicken IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
98 |
91-105 |
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| EDTA plasma (n=4) |
96 |
88-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.487 |
2.570 |
2.529 |
2.454 |
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| 500 |
2.204 |
2.230 |
2.217 |
2.142 |
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| 250 |
1.800 |
1.764 |
1.782 |
1.707 |
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| 125 |
1.390 |
1.394 |
1.392 |
1.317 |
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| 62.5 |
0.917 |
0.950 |
0.934 |
0.859 |
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| 31.2 |
0.584 |
0.568 |
0.576 |
0.501 |
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| 15.6 |
0.348 |
0.362 |
0.355 |
0.280 |
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| 0 |
0.074 |
0.075 |
0.075 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
