Chicken Interleukin 8(IL-8) ELISA Kit
Product Details
| Target Name |
interleukin 8 |
| Alternative Names |
CXCL8 ELISA Kit; CEF4 ELISA Kit; EMF1 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; 9E3 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; CEF-4 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Embryo fibroblast protein 1 ELISA Kit; EMF-1 ELISA Kit |
| Abbreviation |
IL8 |
| Species |
Gallus gallus (Chicken) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
1.56 pg/mL-400 pg/mL |
| Sensitivity |
0.78 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
102 |
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| Range % |
97-106 |
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| 1:2 |
Average % |
88 |
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| Range % |
84-93 |
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| 1:4 |
Average % |
104 |
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| Range % |
99-109 |
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| 1:8 |
Average % |
103 |
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| Range % |
99-106 |
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| Recovery |
| The recovery of chicken IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
94-102 |
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| EDTA plasma (n=4) |
91 |
85-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 400 |
0.187 |
0.183 |
0.185 |
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| 100 |
0.259 |
0.267 |
0.263 |
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| 25 |
0.471 |
0.452 |
0.462 |
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| 6.25 |
0.862 |
0.843 |
0.853 |
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| 1.56 |
1.410 |
1.398 |
1.404 |
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| 0 |
2.251 |
2.159 |
2.205 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
