Dog Matrix metalloproteinase 3,MMP-3 ELISA kit
Product Details
| Target Name |
matrix metallopeptidase 3 (stromelysin 1, progelatinase) |
| Alternative Names |
MMP3Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit |
| Abbreviation |
MMP3 |
| Species |
Canis lupus familiaris (Dog) (Canis familiaris) |
| Sample Types |
serum, plasma, cell culture supernates, urine, tissue homogenates |
| Detection Range |
1.56 ng/mL-100 ng/mL |
| Sensitivity |
0.39 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of dog MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:2 |
Average % |
89 |
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| Range % |
85-96 |
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| 1:4 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:8 |
Average % |
99 |
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| Range % |
92-105 |
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| Recovery |
| The recovery of dog MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
91-99 |
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| EDTA plasma (n=4) |
92 |
87-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.410 |
2.545 |
2.478 |
2.284 |
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| 50 |
1.960 |
1.982 |
1.971 |
1.777 |
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| 25 |
1.595 |
1.563 |
1.579 |
1.385 |
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| 12.5 |
1.177 |
1.201 |
1.189 |
0.995 |
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| 6.25 |
0.786 |
0.789 |
0.788 |
0.594 |
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| 3.12 |
0.458 |
0.462 |
0.460 |
0.266 |
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| 1.56 |
0.278 |
0.262 |
0.270 |
0.076 |
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| 0 |
0.193 |
0.195 |
0.194 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
