Fish growth hormone,GH ELISA Kit
Product Details
| Description |
CUSABIO's product CSB-E12121Fh is a ready-to-use ELISA kit suitable for the measurement of growth hormone (GH) in fish serum, plasma, or tissue homogenates. It can detect fish GH in the range of 312.5 pg/mL-5000 pg/mL. And it has been validated in multiple parameters, including sensitivity, specificity, precision, linearity, and recovery.
The mechanism of this assay is based on the competitive inhibition enzyme immunoassay technique. In this assay, the kit has been pre-coated with an antibody specific to GH. Samples and standards are respectively pipetted into wells with the Biotin-conjugated GH. GH in the sample or in the standards competes with the biotin-labeled GH for binding to the pre-coated GH antibody. After washing, HRP-Avidin conjugate is added to wells. The solution turns blue after the addition of the TMB substrate. The color reaction is terminated and the solution turns from blue to yellow when adding the stop solution. The color intensity is negatively correlated to the amount of GH bound in the initial step and is measure at 450 nm via a microplate reader.
Growth hormone (GH) is a protein hormone synthesized and secreted by the anterior lobe of the pituitary gland. It regulates somatic growth by stimulating the production of the IGF1 in the liver and other organs. It also participates in the modulation of metabolism and entry homeostasis.
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| Target Name |
growth hormone,GH |
| Alternative Names |
gh1 ELISA Kit; Somatotropin-1 ELISA Kit; Growth hormone I ELISA Kit; Somatotropin I ELISA Kit |
| Abbreviation |
GH |
| Species |
Fish |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
312.5 pg/mL-5000 pg/mL |
| Sensitivity |
312.5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Developmental Biology |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of fish GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
91-98 |
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| 1:2 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:4 |
Average % |
102 |
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| Range % |
96-106 |
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| 1:8 |
Average % |
96 |
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| Range % |
92-101 |
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| Recovery |
| The recovery of fish GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
92-98 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 5000 |
0.271 |
0.265 |
0.268 |
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| 2500 |
0.526 |
0.514 |
0.520 |
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| 1250 |
0.887 |
0.863 |
0.875 |
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| 625 |
1.664 |
1.684 |
1.674 |
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| 312.5 |
2.557 |
2.602 |
2.580 |
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| Materials provided |
- A 96-well Assay plate --The 96-well plate has been pre-coated with anti-Fish GH antibody.
- Standard (6 x 1 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- HRP-avidin (1 x 6 ml) --Catalyze the TMB to elicit a chromogenic reaction.
- Biotin-conjugated GH (1 x 6 ml) --Bind to the precoated antibody and act as the detection antigen.
- Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
- Wash Buffer (20x concentrate) (1 x 15 ml) --Wash away unbound or free substances.
- Substrate A (1x 7 ml) --Mix with Sbustrate B.
- Substrate B (1x 7 ml) --Mix with Sbustrate A
- Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
