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Fish Insulin-like growth factors 1,IGF-1 ELISA Kit

Product Details

Target Name Insulin-like growth factors 1,IGF-1
Alternative Names igf1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
Abbreviation IGF-1
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 25 pg/mL-2000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%   
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%   
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-98  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 102  
Range % 98-106  
1:8 Average % 87  
Range % 82-93  
Recovery
The recovery of fish IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-99  
EDTA plasma (n=4) 88 82-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
2000 0.341 0.343 0.342    
750 0.476 0.484 0.480    
375 0.682 0.636 0.659    
125 1.067 1.072 1.070    
25 1.488 1.567 1.528    
0 1.955 1.995 1.975    
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days