Fish Insulin-like growth factors 1,IGF-1 ELISA Kit
Product Details
| Target Name |
Insulin-like growth factors 1,IGF-1 |
| Alternative Names |
igf1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit |
| Abbreviation |
IGF-1 |
| Species |
Fish |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
25 pg/mL-2000 pg/mL |
| Sensitivity |
31.25 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:4 |
Average % |
102 |
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| Range % |
98-106 |
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| 1:8 |
Average % |
87 |
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| Range % |
82-93 |
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| Recovery |
| The recovery of fish IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
90-99 |
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| EDTA plasma (n=4) |
88 |
82-94 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 2000 |
0.341 |
0.343 |
0.342 |
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| 750 |
0.476 |
0.484 |
0.480 |
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| 375 |
0.682 |
0.636 |
0.659 |
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| 125 |
1.067 |
1.072 |
1.070 |
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| 25 |
1.488 |
1.567 |
1.528 |
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| 0 |
1.955 |
1.995 |
1.975 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
