Fish Interleukin 6,IL-6 ELISA Kit
Product Details
| Target Name |
Interleukin 6,IL-6 |
| Alternative Names |
il6Interleukin-6 ELISA Kit; IL-6 ELISA Kit |
| Abbreviation |
IL-6 |
| Species |
Fish |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
12.5 pg/mL-200 pg/mL |
| Sensitivity |
12.5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
88-97 |
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| 1:2 |
Average % |
89 |
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| Range % |
82-94 |
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| 1:4 |
Average % |
103 |
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| Range % |
94-107 |
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| 1:8 |
Average % |
96 |
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| Range % |
90-101 |
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| Recovery |
| The recovery of fish IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
100 |
91-104 |
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| EDTA plasma (n=4) |
91 |
84-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 200 |
0.128 |
0.133 |
0.131 |
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| 100 |
0.269 |
0.258 |
0.264 |
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| 50 |
0.449 |
0.431 |
0.440 |
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| 25 |
0.851 |
0.842 |
0.847 |
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| 12.5 |
1.813 |
1.783 |
1.798 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
