Goat Interleukin 10,IL-10 ELISA Kit
Product Details
| Target Name |
Interleukin 10,IL-10 |
| Abbreviation |
IL10 |
| Species |
Capra hircus (Goat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
82 |
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| Range % |
80-85 |
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| 1:2 |
Average % |
102 |
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| Range % |
97-107 |
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| 1:4 |
Average % |
89 |
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| Range % |
84-93 |
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| 1:8 |
Average % |
107 |
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| Range % |
103-111 |
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| Recovery |
| The recovery of goat IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
90-98 |
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| EDTA plasma (n=4) |
85 |
80-90 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.725 |
2.768 |
2.747 |
2.615 |
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| 1000 |
2.059 |
2.000 |
2.030 |
1.898 |
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| 500 |
1.321 |
1.368 |
1.345 |
1.213 |
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| 250 |
0.741 |
0.758 |
0.750 |
0.618 |
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| 125 |
0.455 |
0.432 |
0.444 |
0.312 |
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| 62.5 |
0.307 |
0.301 |
0.304 |
0.172 |
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| 31.25 |
0.228 |
0.226 |
0.227 |
0.095 |
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| 0 |
0.131 |
0.133 |
0.132 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
