Goat Interleukin 6,IL-6 ELISA Kit
Product Details
| Target Name |
interleukin 6 (interferon, beta 2) |
| Alternative Names |
IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit |
| Abbreviation |
IL6 |
| Species |
Capra hircus (Goat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
1.56 pg/mL-100 pg/mL |
| Sensitivity |
0.39 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-91 |
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| 1:2 |
Average % |
102 |
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| Range % |
97-107 |
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| 1:4 |
Average % |
84 |
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| Range % |
81-89 |
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| 1:8 |
Average % |
99 |
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| Range % |
96-104 |
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| Recovery |
| The recovery of goat IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
90-101 |
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| EDTA plasma (n=4) |
102 |
97-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.625 |
2.698 |
2.662 |
2.542 |
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| 50 |
1.959 |
2.001 |
1.980 |
1.860 |
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| 25 |
1.221 |
1.314 |
1.268 |
1.148 |
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| 12.5 |
0.741 |
0.705 |
0.723 |
0.603 |
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| 6.25 |
0.455 |
0.468 |
0.462 |
0.342 |
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| 3.12 |
0.307 |
0.318 |
0.313 |
0.193 |
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| 1.56 |
0.238 |
0.237 |
0.238 |
0.118 |
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| 0 |
0.121 |
0.119 |
0.120 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
