Guinea pig interleukin 13,IL-13 ELISA Kit
Product Details
| Target Name |
interleukin 13,IL-13 |
| Abbreviation |
IL13 |
| Species |
Cavia porcellus (Guinea pig) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
7.5 pg/mL-300 pg/mL |
| Sensitivity |
3.75 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of Guinea pig IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
83-92 |
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| 1:2 |
Average % |
95 |
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| Range % |
85-105 |
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| 1:4 |
Average % |
93 |
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| Range % |
86-102 |
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| 1:8 |
Average % |
97 |
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| Range % |
92-110 |
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| Recovery |
| The recovery of Guinea pig IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
91-103 |
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| EDTA plasma (n=4) |
93 |
86-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
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| 300 |
2.244 |
2.267 |
2.256 |
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| 150 |
1.173 |
1.161 |
1.167 |
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| 75 |
0.628 |
0.650 |
0.639 |
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| 37.5 |
0.359 |
0.343 |
0.351 |
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| 15 |
0.152 |
0.151 |
0.152 |
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| 7.5 |
0.083 |
0.079 |
0.081 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
