Guinea pig Interleukin 8,IL-8 ELISA Kit
Product Details
| Target Name |
interleukin 8 |
| Alternative Names |
CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Neutrophil attractant protein 1 ELISA Kit; NAP-1 ELISA Kit |
| Abbreviation |
IL8 |
| Species |
Cavia porcellus (Guinea pig) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
90-102 |
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| 1:2 |
Average % |
95 |
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| Range % |
85-100 |
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| 1:4 |
Average % |
95 |
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| Range % |
88-102 |
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| 1:8 |
Average % |
97 |
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| Range % |
90-104 |
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| Recovery |
| The recovery of guinea pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
98 |
95-102 |
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| EDTA plasma (n=4) |
94 |
90-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.253 |
2.245 |
2.249 |
2.134 |
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| 1000 |
1.631 |
1.696 |
1.664 |
1.549 |
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| 500 |
1.111 |
1.135 |
1.123 |
1.008 |
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| 250 |
0.717 |
0.752 |
0.735 |
0.620 |
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| 125 |
0.434 |
0.423 |
0.429 |
0.314 |
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| 62.5 |
0.343 |
0.354 |
0.349 |
0.234 |
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| 31.25 |
0.228 |
0.241 |
0.235 |
0.120 |
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| 0 |
0.116 |
0.113 |
0.115 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
