Hamster Interleukin 6,IL-6 ELISA Kit
Product Details
| Target Name |
Interleukin 6,IL-6 |
| Abbreviation |
IL-6 |
| Species |
Mesocricetus auratus (Golden hamster) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
7.8 pg/mL-500 pg/mL |
| Sensitivity |
1.95 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
82-92 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:4 |
Average % |
96 |
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| Range % |
91-100 |
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| 1:8 |
Average % |
94 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of hamster IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
99 |
94-104 |
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| EDTA plasma (n=4) |
91 |
85-95 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.576 |
2.456 |
2.516 |
2.351 |
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| 250 |
2.107 |
2.076 |
2.092 |
1.927 |
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| 125 |
1.423 |
1.417 |
1.420 |
1.255 |
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| 62.5 |
0.730 |
0.679 |
0.705 |
0.540 |
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| 31.2 |
0.457 |
0.470 |
0.464 |
0.299 |
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| 15.6 |
0.326 |
0.308 |
0.317 |
0.152 |
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| 7.8 |
0.262 |
0.247 |
0.255 |
0.090 |
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| 0 |
0.161 |
0.168 |
0.165 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
