Human 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial (CYP24A1) ELISA kit
Product Details
| Target Name |
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial (CYP24A1)，cytochrome P450, family 24, subfamily A, polypeptide 1 |
| Alternative Names |
1 25 @dihydroxyvitamin D3 24 hydroxylase ELISA Kit; 1 25 dihydroxyvitamin D(3) 24 hydroxylase ELISA Kit; 1 ELISA Kit; 1,25-dihydroxyvitamin D(3) 24-hydroxylase; mitochondrial ELISA Kit; 24 OHase ELISA Kit; 24-OHase ELISA Kit; 25-dihydroxyvitamin D(3) 24-hydroxylase ELISA Kit; CP 24 ELISA Kit; CP24 ELISA Kit; CP24A_HUMAN ELISA Kit; CYP 24 ELISA Kit; CYP24 ELISA Kit; CYP24A1 ELISA Kit; Cytochrome P450 24A1 ELISA Kit; Cytochrome P450 24A1 mitochondrial ELISA Kit; cytochrome P450 CC24 ELISA Kit; Cytochrome P450 family 24 ELISA Kit; Cytochrome P450 family 24 subfamily A member 1 ELISA Kit; Cytochrome P450 family 24 subfamily A polypeptide 1 ELISA Kit; Cytochrome P450 subfamily XXIV ELISA Kit; Cytochrome P450; subfamily XXIV (vitamin D 24-hydroxylase) ELISA Kit; Cytochrome P450-CC24 ELISA Kit; EC 1.14.13.n4 ELISA Kit; Exo mitochondrial protein ELISA Kit; HCAI ELISA Kit; HCINF1 ELISA Kit; MGC126273 ELISA Kit; MGC126274 ELISA Kit; mitochondrial ELISA Kit; P450 CC24 ELISA Kit; Vitamin D 24 hydroxylase ELISA Kit; Vitamin D(3) 24 hydroxylase ELISA Kit; Vitamin D(3) 24-hydroxylase ELISA Kit |
| Abbreviation |
CYP24A1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
7.8 pg/mL-500 pg/mL |
| Sensitivity |
1.95 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYP24A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
86-97 |
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| 1:2 |
Average % |
89 |
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| Range % |
83-94 |
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| 1:4 |
Average % |
97 |
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| Range % |
91-102 |
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| 1:8 |
Average % |
98 |
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| Range % |
92-103 |
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| Recovery |
| The recovery of human CYP24A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
88-99 |
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| EDTA plasma (n=4) |
86 |
80-91 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 500 |
2.719 |
2.612 |
2.666 |
2.534 |
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| 250 |
2.226 |
2.121 |
2.174 |
2.042 |
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| 125 |
1.407 |
1.504 |
1.456 |
1.324 |
|
| 62.5 |
0.845 |
0.821 |
0.833 |
0.701 |
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| 31.2 |
0.457 |
0.466 |
0.462 |
0.330 |
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| 15.6 |
0.323 |
0.314 |
0.319 |
0.187 |
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| 7.8 |
0.221 |
0.226 |
0.224 |
0.092 |
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| 0 |
0.133 |
0.131 |
0.132 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
