Human 17-Hydroxyprogesterone,17-OHP ELISA Kit
Product Details
| Target Name |
17-Hydroxyprogesterone,17-OHP |
| Abbreviation |
17-OHP |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.025 ng/mL-10 ng/mL |
| Sensitivity |
0.025 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Biochemicals |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human 17-OHP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
90-98 |
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| 1:2 |
Average % |
92 |
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| Range % |
89-102 |
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| 1:4 |
Average % |
95 |
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| Range % |
87-102 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-103 |
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| Recovery |
| The recovery of human 17-OHP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-98 |
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| EDTA plasma (n=4) |
94 |
92-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 0 |
2.128 |
2.101 |
2.115 |
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| 0.025 |
1.420 |
1.443 |
1.432 |
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| 0.087 |
0.867 |
0.896 |
0.882 |
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| 0.312 |
0.522 |
0.532 |
0.527 |
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| 1.875 |
0.289 |
0.305 |
0.297 |
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| 10 |
0.149 |
0.155 |
0.152 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
