Human 2,3-Diphosphoglycerate,2,3-DPG ELISA Kit
Product Details
| Target Name |
2,3-Diphosphoglycerate,2,3-DPG |
| Abbreviation |
2,3DPG |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
0.312 μmol/mL-20 μmol/mL |
| Sensitivity |
0.078 μmol/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human 2, 3-DPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
92-100 |
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| 1:2 |
Average % |
98 |
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| Range % |
92-103 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-99 |
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| 1:8 |
Average % |
90 |
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| Range % |
85-96 |
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| Recovery |
| The recovery of human 2, 3-DPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
85-94 |
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| EDTA plasma (n=4) |
84 |
80-89 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| µmol/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.873 |
2.712 |
2.793 |
2.636 |
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| 10 |
2.265 |
2.149 |
2.207 |
2.050 |
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| 5 |
1.372 |
1.328 |
1.350 |
1.193 |
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| 2.5 |
0.854 |
0.813 |
0.834 |
0.677 |
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| 1.25 |
0.457 |
0.481 |
0.469 |
0.312 |
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| 0.625 |
0.337 |
0.357 |
0.347 |
0.190 |
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| 0.312 |
0.202 |
0.207 |
0.205 |
0.048 |
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| 0 |
0.161 |
0.152 |
0.157 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
