Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial(CYP27B1) ELISA kit
Product Details
| Target Name |
cytochrome P450, family 27, subfamily B, polypeptide 1 |
| Alternative Names |
1alpha(OH)ase ELISA Kit; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase ELISA Kit; 25-hydroxyvitamin D-1 alpha hydroxylase ELISA Kit; 25-OHD-1 alpha-hydroxylase ELISA Kit; Calcidiol 1-monooxygenase ELISA Kit; CP27B_HUMAN ELISA Kit; CP2B ELISA Kit; CYP1 ELISA Kit; CYP1ALPHA ELISA Kit; CYP27B ELISA Kit; Cyp27b1 ELISA Kit; Cytochrome p450 27B1 ELISA Kit; Cytochrome p450 27B13 ELISA Kit; Cytochrome P450 family 27 subfamily B polypeptide 1 ELISA Kit; Cytochrome P450 subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase) polypeptide 1 ELISA Kit; Cytochrome P450 subfamily XXVIIB polypeptide 1 ELISA Kit; Cytochrome P450C1 alpha ELISA Kit; Cytochrome P450VD1-alpha ELISA Kit; mitochondrial ELISA Kit; P450C1 alpha ELISA Kit; P450c1 ELISA Kit; P450C1-alpha ELISA Kit; P450VD1-alpha ELISA Kit; PDDR ELISA Kit; VD3 1A hydroxylase ELISA Kit; VDD1 ELISA Kit; VDDR ELISA Kit; VDDR I ELISA Kit; VDDRI ELISA Kit; VDR ELISA Kit |
| Abbreviation |
CYP27B1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYP27B1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
90-97 |
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| 1:2 |
Average % |
89 |
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| Range % |
85-96 |
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| 1:4 |
Average % |
96 |
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| Range % |
90-100 |
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| 1:8 |
Average % |
94 |
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| Range % |
87-97 |
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| Recovery |
| The recovery of human CYP27B1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
85-96 |
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| EDTA plasma (n=4) |
92 |
87-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.472 |
2.372 |
2.422 |
2.248 |
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| 1000 |
1.715 |
1.615 |
1.665 |
1.491 |
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| 500 |
1.088 |
1.048 |
1.068 |
0.894 |
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| 250 |
0.711 |
0.701 |
0.706 |
0.532 |
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| 125 |
0.404 |
0.394 |
0.399 |
0.225 |
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| 62.5 |
0.310 |
0.300 |
0.305 |
0.131 |
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| 31.25 |
0.249 |
0.239 |
0.244 |
0.070 |
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| 0 |
0.175 |
0.173 |
0.174 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
