Human 3-nitrotyrosine (3-NT) ELISA Kit
Product Details
| Target Name |
3-nitrotyrosine (3-NT) |
| Abbreviation |
3-NT |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human 3-NT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
92-102 |
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| 1:2 |
Average % |
90 |
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| Range % |
82-98 |
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| 1:4 |
Average % |
104 |
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| Range % |
99-111 |
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| 1:8 |
Average % |
86 |
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| Range % |
82-96 |
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| Recovery |
| The recovery of human 3-NT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
85-97 |
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| EDTA plasma (n=4) |
91 |
85-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.655 |
2.752 |
2.704 |
2.553 |
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| 5 |
2.111 |
2.275 |
2.193 |
2.042 |
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| 2.5 |
1.486 |
1.465 |
1.476 |
1.325 |
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| 1.25 |
0.769 |
0.799 |
0.784 |
0.633 |
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| 0.625 |
0.485 |
0.462 |
0.474 |
0.323 |
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| 0.312 |
0.372 |
0.399 |
0.386 |
0.235 |
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| 0.156 |
0.251 |
0.264 |
0.258 |
0.107 |
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| 0 |
0.152 |
0.150 |
0.151 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
