Human 3-oxo-5-alpha-steroid 4-dehydrogenase 1(SRD5A1) ELISA kit
Product Details
| Target Name |
steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) |
| Alternative Names |
3 oxo 5 alpha steroid 4 dehydrogenase 1 ELISA Kit; 3 oxo 5 alpha steroid delta 4 dehydrogenase alpha 1 ELISA Kit; 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1,5-alpha reductase ELISA Kit; 3-oxo-5-alpha-steroid 4-dehydrogenase 1 ELISA Kit; 5 alpha reductase ELISA Kit; S5A1_HUMAN ELISA Kit; S5AR 1 ELISA Kit; S5AR ELISA Kit; SR type 1 ELISA Kit; SRD5A 1 ELISA Kit; Srd5a1 ELISA Kit; Steroid 5 alpha reductase 1 ELISA Kit; Steroid 5 alpha reductase alpha polypeptide 1 (3 oxo 5 alpha steroid delta 4 dehydrogenase alpha 1) ELISA Kit; Steroid 5 alpha reductase alpha polypeptide 1 ELISA Kit; Steroid 5 alpha reductase type I ELISA Kit; Steroid 5-alpha-reductase 1 ELISA Kit |
| Abbreviation |
SRD5A1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human SRD5A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
90-102 |
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| 1:2 |
Average % |
90 |
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| Range % |
84-95 |
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| 1:4 |
Average % |
101 |
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| Range % |
95-106 |
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| 1:8 |
Average % |
98 |
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| Range % |
92-104 |
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| Recovery |
| The recovery of human SRD5A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
85-93 |
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| EDTA plasma (n=4) |
92 |
87-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 10 |
2.017 |
2.063 |
2.040 |
1.795 |
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| 5 |
1.499 |
1.472 |
1.486 |
1.241 |
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| 2.5 |
1.004 |
1.064 |
1.034 |
0.789 |
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| 1.25 |
0.705 |
0.742 |
0.724 |
0.479 |
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| 0.625 |
0.501 |
0.536 |
0.519 |
0.274 |
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| 0.312 |
0.412 |
0.438 |
0.425 |
0.180 |
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| 0.156 |
0.347 |
0.361 |
0.354 |
0.109 |
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| 0 |
0.241 |
0.249 |
0.245 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
