Human 5-hydroxyeicosatetraenoic acid(5-HETE)ELISA Kit
Product Details
| Target Name |
5-hydroxyeicosatetraenoic acid(5-HETE) |
| Abbreviation |
5-HETE |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Biochemicals |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HETE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
89 |
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| Range % |
84-94 |
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| 1:200 |
Average % |
92 |
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| Range % |
87-99 |
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| 1:400 |
Average % |
93 |
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| Range % |
85-97 |
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| 1:800 |
Average % |
97 |
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| Range % |
90-104 |
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| Recovery |
| The recovery of human 5-HETE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
87-97 |
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| EDTA plasma (n=4) |
91 |
84-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.739 |
2.652 |
2.696 |
2.586 |
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| 1000 |
2.258 |
2.176 |
2.217 |
2.107 |
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| 500 |
1.513 |
1.504 |
1.509 |
1.399 |
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| 250 |
0.805 |
0.742 |
0.774 |
0.664 |
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| 125 |
0.434 |
0.413 |
0.424 |
0.314 |
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| 62.5 |
0.275 |
0.261 |
0.268 |
0.158 |
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| 31.25 |
0.182 |
0.179 |
0.181 |
0.071 |
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| 0 |
0.112 |
0.107 |
0.110 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
