Human 5-Hydroxytryptamine,5HT ELISA Kit
Product Details
| Target Name |
5-Hydroxytryptamine,5HT |
| Abbreviation |
5HT |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
4 ng/mL-800 ng/mL |
| Sensitivity |
2 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
92-102 |
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| 1:2 |
Average % |
94 |
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| Range % |
91-98 |
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| 1:4 |
Average % |
85 |
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| Range % |
80-89 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-99 |
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| Recovery |
| The recovery of human 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
90-99 |
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| EDTA plasma (n=4) |
89 |
85-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 800 |
0.215 |
0.216 |
0.216 |
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| 200 |
0.383 |
0.391 |
0.387 |
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| 64 |
0.575 |
0.567 |
0.571 |
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| 16 |
0.986 |
0.974 |
0.980 |
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| 4 |
1.571 |
1.472 |
1.522 |
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| 0 |
2.444 |
2.345 |
2.395 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
