Human A disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS5) ELISA kit
Product Details
| Target Name |
ADAM metallopeptidase with thrombospondin type 1 motif, 5 |
| Alternative Names |
A disintegrin and metalloproteinase with thrombospondin motifs 11 ELISA Kit; A disintegrin and metalloproteinase with thrombospondin motifs 5 ELISA Kit; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 ELISA Kit; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase 2) ELISA Kit; A disintegrin-like and metalloprotease with thrombospondin type 1 motif, 5 ELISA Kit; A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 ELISA Kit; ADAM metallopeptidase with thrombospondin type 1 motif 5 ELISA Kit; ADAM TS 11 ELISA Kit; ADAM TS 5 ELISA Kit; ADAM TS5 ELISA Kit; ADAM-TS 11 ELISA Kit; ADAM-TS 5 ELISA Kit; ADAM-TS5 ELISA Kit; ADAMTS 11 ELISA Kit; ADAMTS 5 ELISA Kit; ADAMTS-11 ELISA Kit; ADAMTS-5 ELISA Kit; ADAMTS11 ELISA Kit; ADAMTS11, formerly ELISA Kit; Adamts5 ELISA Kit; ADMP 2 ELISA Kit; ADMP-2 ELISA Kit; ADMP2 ELISA Kit; Aggrecanase 2 ELISA Kit; Aggrecanase-2 ELISA Kit; ATS5_HUMAN ELISA Kit; FLJ36738 ELISA Kit; Implantin ELISA Kit; ThromboSpondin motif-5 ELISA Kit |
| Abbreviation |
ADAMTS5 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6 ng/mL-1000 ng/mL |
| Sensitivity |
3.9 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
89-94 |
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| 1:2 |
Average % |
104 |
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| Range % |
100-107 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-95 |
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| 1:8 |
Average % |
98 |
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| Range % |
93-101 |
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| Recovery |
| The recovery of human ADAMTS5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
89-99 |
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| EDTA plasma (n=4) |
89 |
82-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.154 |
2.254 |
2.204 |
2.062 |
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| 500 |
1.512 |
1.612 |
1.562 |
1.420 |
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| 250 |
1.001 |
1.011 |
1.006 |
0.864 |
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| 125 |
0.536 |
0.546 |
0.541 |
0.399 |
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| 62.5 |
0.328 |
0.328 |
0.328 |
0.186 |
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| 31.25 |
0.257 |
0.267 |
0.262 |
0.120 |
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| 15.6 |
0.189 |
0.199 |
0.194 |
0.052 |
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| 0 |
0.142 |
0.141 |
0.142 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
