Human Acetyl-CoA carboxylase 1(ACACA) ELISA kit
Product Details
| Target Name |
acetyl-Coenzyme A carboxylase alpha |
| Alternative Names |
ACAC ELISA Kit; ACACA ELISA Kit; ACACA_HUMAN ELISA Kit; ACACB ELISA Kit; ACC alpha ELISA Kit; ACC ELISA Kit; ACC beta ELISA Kit; ACC-alpha ELISA Kit; ACC1 ELISA Kit; ACC2 ELISA Kit; ACCA ELISA Kit; ACCB ELISA Kit; Acetyl CoA carboxylase 1 ELISA Kit; Acetyl CoA carboxylase 2 ELISA Kit; Acetyl CoA carboxylase alpha ELISA Kit; Acetyl CoA carboxylase beta ELISA Kit; Acetyl Coenzyme A carboxylase alpha ELISA Kit; Acetyl Coenzyme A carboxylase beta ELISA Kit; Biotin carboxylase ELISA Kit; COA1 ELISA Kit; COA2 ELISA Kit; HACC275 ELISA Kit; OTTHUMP00000164069 ELISA Kit; OTTHUMP00000164070 ELISA Kit; OTTHUMP00000164076 ELISA Kit; OTTHUMP00000240532 ELISA Kit |
| Abbreviation |
ACACA |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
9.38 pg/mL-600 pg/mL |
| Sensitivity |
2.34 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ACACA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
98 |
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| Range % |
96-102 |
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| 1:10 |
Average % |
107 |
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| Range % |
103-114 |
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| 1:20 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:40 |
Average % |
88 |
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| Range % |
86-95 |
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| Recovery |
| The recovery of human ACACA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
105 |
99-108 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 600 |
2.169 |
2.202 |
2.186 |
2.004 |
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| 300 |
1.702 |
1.814 |
1.758 |
1.576 |
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| 150 |
1.202 |
1.254 |
1.228 |
1.046 |
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| 75 |
0.711 |
0.747 |
0.729 |
0.547 |
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| 37.5 |
0.503 |
0.506 |
0.505 |
0.323 |
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| 18.75 |
0.284 |
0.298 |
0.291 |
0.109 |
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| 9.38 |
0.236 |
0.249 |
0.243 |
0.061 |
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| 0 |
0.174 |
0.189 |
0.182 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
