Human ADAM metallopeptidase with thrombospondin type 1 motif4,ADAMTS4 ELISA Kit
Product Details
| Target Name |
ADAM metallopeptidase with thrombospondin type 1 motif, 4 |
| Alternative Names |
A disintegrin and metalloproteinase with thrombospondin motifs 4 ELISA Kit; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4 ELISA Kit; A disintegrin like and metalloprotease with thrombospondin type 1 motif 4 ELISA Kit; ADAM metallopeptidase with thrombospondin type 1 motif 4 ELISA Kit; ADAM TS 4 ELISA Kit; ADAM TS4 ELISA Kit; ADAM-TS 4 ELISA Kit; ADAM-TS4 ELISA Kit; ADAMTS 2 ELISA Kit; ADAMTS 4 ELISA Kit; ADAMTS-4 ELISA Kit; ADAMTS2 ELISA Kit; ADAMTS4 ELISA Kit; ADAMTS4 protein ELISA Kit; ADMP 1 ELISA Kit; ADMP-1 ELISA Kit; ADMP1 ELISA Kit; Aggrecanase 1 ELISA Kit; Aggrecanase-1 ELISA Kit; Aggrecanase1 ELISA Kit; ATS4_HUMAN ELISA Kit; KIAA0688 ELISA Kit |
| Abbreviation |
ADAMTS4 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, urine, tissue homogenates, cell lysates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:200 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:400 |
Average % |
97 |
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| Range % |
92-115 |
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| 1:800 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human ADAMTS4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
90-101 |
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| EDTA plasma (n=4) |
97 |
90-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.748 |
2.801 |
2.775 |
2.685 |
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| 500 |
2.235 |
2.241 |
2.238 |
2.148 |
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| 250 |
1.542 |
1.567 |
1.555 |
1.465 |
|
| 125 |
0.906 |
0.943 |
0.925 |
0.835 |
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| 62.5 |
0.446 |
0.467 |
0.457 |
0.367 |
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| 31.2 |
0.243 |
0.264 |
0.254 |
0.164 |
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| 15.6 |
0.152 |
0.149 |
0.151 |
0.061 |
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| 0 |
0.092 |
0.087 |
0.090 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
