Human Adenylyl cyclase-associated protein 1(CAP1) ELISA kit
Product Details
| Target Name |
CAP, adenylate cyclase-associated protein 1 (yeast) |
| Alternative Names |
Adenylyl cyclase associated protein 1 ELISA Kit; Adenylyl cyclase-associated protein 1 ELISA Kit; CAP 1 ELISA Kit; CAP ELISA Kit; CAP; adenylate cyclase associated protein 1 (yeast) ELISA Kit; CAP1 ELISA Kit; CAP1 PEN ELISA Kit; CAP1-PEN ELISA Kit; CAP1_HUMAN ELISA Kit |
| Abbreviation |
CAP1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
25 pg/mL-1600 pg/mL |
| Sensitivity |
6.25 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CAP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
87-97 |
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| 1:2 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:4 |
Average % |
104 |
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| Range % |
100-107 |
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| 1:8 |
Average % |
95 |
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| Range % |
92-99 |
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| Recovery |
| The recovery of human CAP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
88 |
84-93 |
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| EDTA plasma (n=4) |
92 |
88-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1600 |
2.104 |
2.268 |
2.186 |
2.083 |
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| 800 |
1.578 |
1.590 |
1.584 |
1.481 |
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| 400 |
1.163 |
1.152 |
1.158 |
1.055 |
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| 200 |
0.707 |
0.727 |
0.717 |
0.614 |
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| 100 |
0.495 |
0.474 |
0.485 |
0.382 |
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| 50 |
0.292 |
0.278 |
0.285 |
0.182 |
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| 25 |
0.196 |
0.189 |
0.193 |
0.090 |
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| 0 |
0.102 |
0.104 |
0.103 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
