Human Adipose Triglyceride Lipase,ATGL ELISA kit
Product Details
| Target Name |
patatin-like phospholipase domain containing 2 |
| Alternative Names |
1110001C14Rik ELISA Kit; Adipose triglyceride lipase ELISA Kit; ATGL ELISA Kit; ATGL DESNUTRIN ELISA Kit; Calcium independent phospholipase A2 ELISA Kit; Calcium-independent phospholipase A2 ELISA Kit; Desnutrin ELISA Kit; EC 3.1.1.3 ELISA Kit; FP17548 ELISA Kit; IPLA2 zeta ELISA Kit; IPLA2-zeta ELISA Kit; Mutant patatin like phospholipase domain containing 2 ELISA Kit; Patatin like phospholipase domain containing 2 ELISA Kit; PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2 ELISA Kit; Patatin-like phospholipase domain-containing protein 2 ELISA Kit; PEDF R ELISA Kit; PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA ELISA Kit; Pigment epithelium derived factor ELISA Kit; Pigment epithelium-derived factor ELISA Kit; plpl ELISA Kit; plpl2 ELISA Kit; PLPL2_HUMAN ELISA Kit; Pnpla2 ELISA Kit; Transport secretion protein 2 ELISA Kit; Transport secretion protein 2.2 ELISA Kit; Transport-secretion protein 2 ELISA Kit; Triglyceride hydrolase ELISA Kit; TTS 2.2 ELISA Kit; TTS2 ELISA Kit; TTS2.2 ELISA Kit; ZETA ELISA Kit |
| Abbreviation |
PNPLA2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, urine, tissue homogenates |
| Detection Range |
6.25 mIU/mL-400 mIU/mL |
| Sensitivity |
1.56 mIU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ATGL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-95 |
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| 1:2 |
Average % |
96 |
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| Range % |
90-100 |
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| 1:4 |
Average % |
93 |
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| Range % |
88-105 |
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| 1:8 |
Average % |
95 |
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| Range % |
82-110 |
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| Recovery |
| The recovery of human ATGL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
82-103 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mIU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.083 |
1.992 |
2.038 |
1.916 |
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| 200 |
1.854 |
1.796 |
1.825 |
1.703 |
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| 100 |
1.529 |
1.484 |
1.507 |
1.385 |
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| 50 |
1.038 |
0.976 |
1.007 |
0.885 |
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| 25 |
0.771 |
0.709 |
0.740 |
0.618 |
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| 12.5 |
0.429 |
0.395 |
0.412 |
0.290 |
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| 6.25 |
0.286 |
0.269 |
0.278 |
0.156 |
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| 0 |
0.124 |
0.119 |
0.122 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
