Human alanyl (membrane) aminopeptidase (ANPEP)ELISA kit
Product Details
| Target Name |
alanyl (membrane) aminopeptidase |
| Alternative Names |
Alanyl (membrane) aminopeptidase ELISA Kit; Alanyl aminopeptidase ELISA Kit; Aminopeptidase M ELISA Kit; Aminopeptidase N ELISA Kit; AMPN_HUMAN ELISA Kit; ANPEP ELISA Kit; AP M ELISA Kit; AP N ELISA Kit; AP-M ELISA Kit; AP-N ELISA Kit; APN ELISA Kit; CD 13 ELISA Kit; CD13 ELISA Kit; CD13 antigen ELISA Kit; gp150 ELISA Kit; hAPN ELISA Kit; LAP 1 ELISA Kit; LAP1 ELISA Kit; Microsomal aminopeptidase ELISA Kit; Myeloid plasma membrane glycoprotein CD13 ELISA Kit; p150 ELISA Kit; PEPN ELISA Kit |
| Abbreviation |
ANPEP |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ANPEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
91 |
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| Range % |
84-99 |
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| 1:40 |
Average % |
91 |
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| Range % |
85-96 |
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| 1:80 |
Average % |
96 |
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| Range % |
92-103 |
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| 1:160 |
Average % |
103 |
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| Range % |
96-109 |
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| Recovery |
| The recovery of human ANPEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
88-97 |
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| EDTA plasma (n=4) |
96 |
90-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.627 |
2.645 |
2.636 |
2.545 |
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| 1000 |
2.131 |
2.298 |
2.215 |
2.124 |
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| 500 |
1.458 |
1.502 |
1.480 |
1.389 |
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| 250 |
0.731 |
0.755 |
0.743 |
0.652 |
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| 125 |
0.377 |
0.352 |
0.365 |
0.274 |
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| 62.5 |
0.201 |
0.212 |
0.207 |
0.116 |
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| 31.25 |
0.189 |
0.188 |
0.189 |
0.098 |
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| 0 |
0.087 |
0.094 |
0.091 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
