Human Allograft inflammatory factor 1(AIF1) ELISA kit
Product Details
| Target Name |
allograft inflammatory factor 1 |
| Alternative Names |
AIF 1 ELISA Kit; AIF-1 ELISA Kit; Aif1 ELISA Kit; AIF1 protein ELISA Kit; AIF1_HUMAN ELISA Kit; Allograft inflammatory factor 1 ELISA Kit; Allograft inflammatory factor 1 splice variant G ELISA Kit; allograft inflammatory factor-1 splice variant Hara-1 ELISA Kit; balloon angioplasty responsive transcription ELISA Kit; BART 1 ELISA Kit; G1 ELISA Kit; G1 putative splice variant of allograft inflamatory factor 1 ELISA Kit; IBA 1 ELISA Kit; IBA1 ELISA Kit; interferon gamma responsive transcript ELISA Kit; Interferon responsive transcript 1 ELISA Kit; interferon responsive transcript factor 1 ELISA Kit; Ionized calcium binding adapter molecule 1 ELISA Kit; Ionized calcium-binding adapter molecule 1 ELISA Kit; ionized calcium-binding adapter molecule ELISA Kit; IRT 1 ELISA Kit; IRT1 ELISA Kit; Microglia response factor ELISA Kit; MRF1 ELISA Kit; Protein g1 ELISA Kit |
| Abbreviation |
AIF1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
7.8 pg/mL-500 pg/mL |
| Sensitivity |
1.9 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human AIF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
104 |
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| Range % |
101-108 |
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| 1:10 |
Average % |
91 |
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| Range % |
88-97 |
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| 1:20 |
Average % |
96 |
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| Range % |
91-104 |
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| 1:40 |
Average % |
89 |
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| Range % |
86-93 |
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| Recovery |
| The recovery of human AIF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
102 |
99-106 |
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| EDTA plasma (n=4) |
96 |
92-101 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.532 |
2.522 |
2.527 |
2.347 |
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| 250 |
2.088 |
2.027 |
2.058 |
1.878 |
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| 125 |
1.369 |
1.459 |
1.414 |
1.234 |
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| 62.5 |
0.784 |
0.779 |
0.782 |
0.602 |
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| 31.2 |
0.445 |
0.480 |
0.463 |
0.283 |
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| 15.6 |
0.287 |
0.299 |
0.293 |
0.113 |
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| 7.8 |
0.213 |
0.230 |
0.222 |
0.042 |
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| 0 |
0.175 |
0.185 |
0.180 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
