Human alpha-1-microglobulin/bikunin precursor,AMBP ELISA Kit
Product Details
| Target Name |
alpha-1-microglobulin/bikunin precursor |
| Alternative Names |
A1M ELISA Kit; Alpha 1 microglobulin/bikunin precursor ELISA Kit; Alpha 1 microglycoprotein ELISA Kit; Alpha-1 microglycoprotein ELISA Kit; Alpha-1-microglobulin ELISA Kit; AMBP ELISA Kit; AMBP_HUMAN ELISA Kit; Bikunin ELISA Kit; Complex-forming glycoprotein heterogeneous in charge ELISA Kit; EDC1 ELISA Kit; Growth inhibiting protein 19 ELISA Kit; HCP ELISA Kit; HI-30 ELISA Kit; HI30 ELISA Kit; IATIL ELISA Kit; ITI ELISA Kit; ITI-LC ELISA Kit; ITIL ELISA Kit; Microglobulin (alpha 1) ELISA Kit; Protein AMBP ELISA Kit; Protein HC ELISA Kit; Trypstatin ELISA Kit; Uristatin ELISA Kit; Uronic-acid-rich protein ELISA Kit; UTI ELISA Kit |
| Abbreviation |
AMBP |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, urine, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1000 |
Average % |
91 |
|
| Range % |
86-98 |
|
| 1:2000 |
Average % |
97 |
|
| Range % |
92-104 |
|
| 1:4000 |
Average % |
85 |
|
| Range % |
81-88 |
|
| 1:8000 |
Average % |
89 |
|
| Range % |
83-96 |
|
|
| Recovery |
| The recovery of human AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
87-102 |
|
| EDTA plasma (n=4) |
96 |
91-101 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 10 |
2.862 |
2.793 |
2.828 |
2.679 |
|
| 5 |
2.531 |
2.567 |
2.549 |
2.400 |
|
| 2.5 |
2.002 |
2.054 |
2.028 |
1.879 |
|
| 1.25 |
1.296 |
1.315 |
1.306 |
1.157 |
|
| 0.625 |
0.643 |
0.672 |
0.658 |
0.509 |
|
| 0.312 |
0.441 |
0.453 |
0.447 |
0.298 |
|
| 0.156 |
0.292 |
0.300 |
0.296 |
0.147 |
|
| 0 |
0.147 |
0.151 |
0.149 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
