Human Alpha-crystallin B chain(CRYAB) ELISA kit
Product Details
| Target Name |
crystallin, alpha B |
| Alternative Names |
AACRYA ELISA Kit; Alpha B crystallin ELISA Kit; Alpha crystallin B chain ELISA Kit; Alpha(B)-crystallin ELISA Kit; Alpha-crystallin B chain ELISA Kit; CRYA2 ELISA Kit; Cryab ELISA Kit; CRYAB_HUMAN ELISA Kit; Crystallin alpha B ELISA Kit; Crystallin alpha polypeptide 2 ELISA Kit; CTPP2 ELISA Kit; Heat shock 20 kD like protein ELISA Kit; Heat shock protein beta 5 ELISA Kit; Heat shock protein beta-5 ELISA Kit; HspB5 ELISA Kit; Renal carcinoma antigen NY REN 27 ELISA Kit; Renal carcinoma antigen NY-REN-27 ELISA Kit; Rosenthal fiber component ELISA Kit |
| Abbreviation |
CRYAB |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
12.5 pg/mL-800 pg/mL |
| Sensitivity |
3.12 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CRYAB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
85-86 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-101 |
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| 1:4 |
Average % |
89 |
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| Range % |
84-93 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-99 |
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| Recovery |
| The recovery of human CRYAB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
89-101 |
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| EDTA plasma (n=4) |
84 |
80-88 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.456 |
2.327 |
2.392 |
2.299 |
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| 400 |
1.689 |
1.621 |
1.655 |
1.562 |
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| 200 |
1.052 |
1.013 |
1.033 |
0.940 |
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| 100 |
0.497 |
0.507 |
0.502 |
0.409 |
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| 50 |
0.304 |
0.329 |
0.317 |
0.224 |
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| 25 |
0.181 |
0.197 |
0.189 |
0.096 |
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| 12.5 |
0.149 |
0.140 |
0.145 |
0.052 |
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| 0 |
0.095 |
0.091 |
0.093 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
