Human alzheimer-associated neuronal thread protein (AD7C-NTP) ELISA kit
Product Details
| Target Name |
alzheimer-associated neuronal thread protein (AD7C-NTP) |
| Abbreviation |
AD7C-NTP |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
93.75 pg/mL-6000 pg/mL |
| Sensitivity |
23.43 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of Human AD7C-NTP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
97 |
|
| Range % |
93-101 |
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| 1:40 |
Average % |
93 |
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| Range % |
86-97 |
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| 1:80 |
Average % |
93 |
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| Range % |
87-97 |
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| 1:160 |
Average % |
102 |
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| Range % |
98-106 |
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| Recovery |
| The recovery of Human AD7C-NTP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
98 |
94-102 |
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| EDTA plasma (n=4) |
87 |
82-93 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 6000 |
2.615 |
2.787 |
2.701 |
2.614 |
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| 3000 |
2.214 |
2.115 |
2.165 |
2.078 |
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| 1500 |
1.441 |
1.386 |
1.414 |
1.327 |
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| 750 |
0.784 |
0.821 |
0.803 |
0.716 |
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| 375 |
0.466 |
0.457 |
0.462 |
0.375 |
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| 187.5 |
0.274 |
0.286 |
0.280 |
0.193 |
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| 93.75 |
0.172 |
0.166 |
0.169 |
0.082 |
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| 0 |
0.086 |
0.087 |
0.087 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
