Human Angiogenin,ANG ELISA Kit
Product Details
| Target Name |
angiogenin, ribonuclease, RNase A family, 5 |
| Alternative Names |
ALS9 ELISA Kit; AMYOTROPHIC LATERAL SCLEROSIS ELISA Kit; ANG 1 ELISA Kit; ANG ELISA Kit; ANG I ELISA Kit; ANG1 ELISA Kit; ANGI ELISA Kit; ANGI_HUMAN ELISA Kit; Angiogenin ELISA Kit; Angiogenin ribonuclease RNase A family; 5 ELISA Kit; Epididymis luminal protein 168 ELISA Kit; HEL168 ELISA Kit; MGC22466 ELISA Kit; MGC71966 ELISA Kit; Ribonuclease 5 ELISA Kit; Ribonuclease RNase A Family 5 ELISA Kit; RNase 5 ELISA Kit; RNASE4 ELISA Kit; RNASE5 ELISA Kit |
| Abbreviation |
ANG |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, urine, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ANG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:200 |
Average % |
88 |
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| Range % |
84-92 |
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| 1:400 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:800 |
Average % |
99 |
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| Range % |
95-104 |
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| 1:1600 |
Average % |
102 |
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| Range % |
96-108 |
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| Recovery |
| The recovery of human ANG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
93-96 |
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| EDTA plasma (n=4) |
100 |
95-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1000 |
1.865 |
1.830 |
1.848 |
1.686 |
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| 500 |
1.457 |
1.446 |
1.452 |
1.290 |
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| 250 |
1.128 |
1.082 |
1.105 |
0.943 |
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| 125 |
0.693 |
0.708 |
0.701 |
0.539 |
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| 62.5 |
0.437 |
0.409 |
0.423 |
0.261 |
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| 31.2 |
0.260 |
0.243 |
0.252 |
0.090 |
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| 15.6 |
0.209 |
0.201 |
0.205 |
0.043 |
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| 0 |
0.163 |
0.160 |
0.162 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
