Human angiotensinogen (aGT) ELISA Kit
Product Details
| Target Name |
angiotensinogen (serpin peptidase inhibitor, clade A, member 8) |
| Alternative Names |
Aangiotensinogen (serpin peptidase inhibitor clade A member 8) ELISA Kit; AGT ELISA Kit; AI265500 ELISA Kit; Alpha 1 antiproteinase antitrypsin ELISA Kit; Ang ELISA Kit; Ang I ELISA Kit; Ang II ELISA Kit; Ang III ELISA Kit; AngII ELISA Kit; Angiotensin I ELISA Kit; Angiotensin II ELISA Kit; Angiotensin III ELISA Kit; Angiotensin-3 ELISA Kit; Angiotensinogen (PAT) ELISA Kit; Angiotensinogen ELISA Kit; ANGT_HUMAN ELISA Kit; ANHU ELISA Kit; ANRT ELISA Kit; AT-2 ELISA Kit; AT-II ELISA Kit; Des-Asp[1]-angiotensin II ELISA Kit; FLJ92595 ELISA Kit; FLJ97926 ELISA Kit; MGC105326 ELISA Kit; PAT ELISA Kit; Pre angiotensinogen ELISA Kit; Serine (or cysteine) proteinase inhibitor ELISA Kit; Serpin A8 ELISA Kit; Serpin peptidase inhibitor clade A member 8 ELISA Kit; SERPINA8 ELISA Kit |
| Abbreviation |
AGT |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, urine,cell culture supernates |
| Detection Range |
78 pg/mL-5000 pg/mL |
| Sensitivity |
19.5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human aGT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
90 |
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| Range % |
85-100 |
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| 1:400 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:800 |
Average % |
98 |
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| Range % |
93-110 |
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| 1:1600 |
Average % |
93 |
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| Range % |
85-105 |
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| Recovery |
| The recovery of human aGT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
85-100 |
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| EDTA plasma (n=4) |
97 |
90-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
2.533 |
2.589 |
2.561 |
2.467 |
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| 2500 |
2.234 |
2.268 |
2.251 |
2.157 |
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| 1250 |
1.618 |
1.628 |
1.623 |
1.529 |
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| 625 |
1.005 |
1.014 |
1.010 |
0.916 |
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| 312 |
0.423 |
0.453 |
0.438 |
0.344 |
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| 156 |
0.236 |
0.243 |
0.240 |
0.146 |
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| 78 |
0.149 |
0.156 |
0.153 |
0.059 |
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| 0 |
0.091 |
0.096 |
0.094 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
